Abstract: The objective of this work was to investigate the bacterial association to remediate diesel oil. We reported the biotic and abiotic contributions to difference in diesel oil degradation efficacy between two bacterial strain Pseudomonas auerigonsa (AHJ8) and Acinetobacter lwoffii (AHJ6) isolated from diesel oil contaminated soil and garden soil. We characterized the physical variations in bioremediation efficacy for diesel oil. Diesel oil and them constitutes as sole carbon sources, quantify the components which they degrade. Initially isolation was carried the detection of hydroxyl metabolite catechol during degradation of diesel oil.