Abstract: The present work assessed the genetic divergence among the genotypes of Costus specious collected from five blocks of Balaghat district of M.P., India by RAPD (Randomly Amplified Polymorphic DNA) using Seventeen random declaimer primers as the plant was found to adored its therapeutic efficacy in Ayurveda and traditional system of medicines and is most important medicinal plants used in Arthritis. A deprogram was constructed for cluster analysis using an un-weighted pair group method with arithmetic means (UPGMA) grouped the genotypes into 2 major clusters based on win boot. Out of the seventeen random primers used for studying genetic divergence, twelve primers were found to be polymorphic. Out of 17 primers, 1 was found to be 100% polymorphic generating a total of 21 alleles with an average of 4.2 products per polymorphic primer. The total numbers of alleles were amplified 393 and percent polymorphic was 43.36 for tall seventeen markers. Genetic similarity/dissimilarity among genotypes was evaluated by generating a similarity matrix based on Jaccard’s co-efficient ranging from 56 to 0.70. Results showed that both environmental and genetic factors were constrained in observing variations. The degree of genetic variations detected among the accessions of Costus specious suggested that RAPD marker advent seemed to be best suited for assessing with high accuracy the genetic relationships among distinct Costus specious accessions.