Abstract: Laboratory investigations were conducted to identify suitable form and to determine the suitable dose of application of osmolytes for liquid inoculant formulations of plant growth promoting rhizobacteria (PGPR) viz., Azotobacter sp., Azospirillum sp., Acinetobacter sp., Bacillus sp. and Pseudomonas sp. Osmolytes such as four grades of polyvinyl pyrrollidone (PVP), four grades of polyethylene glycol (PEG), and glycerol were added to broth at three different concentrations (0.5%, 1.0% and 2.0%). Supplementing the specific media with 2 % Glycerol for Azotobacter sp., 2 % PVP K-15 for Bacillus sp., 1 % PEG 400 for Azospirillum sp., 2 % PVP K-15 for Pseudomonas sp., and 2 % PEG 4000 for Acinetobacter sp. resulted in the highest population densities.