Abstract: Flexibility is an essential fitness component that decrease with age and immobility can lead to change in muscle and joint function postural alignment and host of indirect impairments like muscle tightness , atrophy, fibrosis, contracture, joint analysis, postural deformity. Tight hamstring muscle may be a potential risk factor for strain injuries in sports, in which a full range of motion is needed. The stiff and short hamstring muscle tendon unit may be a risk factor for low back pain and the development of patellar tendinopathy. At present various interventions are available to increase flexibility of hamstring muscle. However, there are not many studies that have analyzed the effects of static stretching and proprioceptive neuromuscular facilitation stretching of hamstring muscles following superficial heat in athletes. The aim of the study was to find out the efficacy of effectiveness of static stretching and PNF stretching of hamstring muscles following superficial heat in athletes.