Abstract: The present investigations were conducted to evaluate the effect of ovatide treatment on the reproduction in Puntius sophore. Hormone was injected intramuscularly @ 4mg/kg and 0.5mg/kg body weight fortnightly for 90 days in two phases. Phase I was initiated at stage I (Resting stage) and Phase II at stage II(pre-maturing stage) of ovarian maturation. Results reveals that hormonal treatment induce ovarian maturation, stimulate ovulation, and expressed an advancement in time of spawning. It was revealed that spawning could be advanced by four months if treatment occur at stage I and by three months if treatment occur at stage II of the reproductive cycle.