Abstract: Gastric ulcer is one of the most prevalent gastrointestinal disorders, which affects approximately 5-10% of people during their life. In recent years, abundant work has been carried out on herbal medicine to clarify their potential efficacy in gastric ulcer prevention or management. Here, the present study was carried out to investigate antiulcer activity of ethanolic extract of Madhuca longifolia flowers in pylorus ligated ulceration in the albino rats .The ethanolic extract of Madhuca longifolia flowers at doses of 100,200,300 mg/kg b.w produced significant (p<0.01) inhibition of the gastric fluid volume, free acidity, total acidity. In conclusion the antiulcer properties of the extract may be attributed to the presence of phytochemicals like flavonoids (quercetin), alkaloids and tannins present in the plant extract with various biological activities.