Abstract: This study examined healthcare workers perceptions of centralized electronic medical records (EMR) for HIV care and treatment in Uasin Gishu County, Kenya. Human Immunodeficiency Virus (HIV) remains a significant public health challenge in Africa and Kenya whereby existing electronic medical records (EMRs) for HIV care majorly operate as standalone systems, leading to duplication, lack of interoperability, and inconsistent documentation or unrecorded diagnoses, limited adherence tracking, and unreliable patient data management. Limited research has explored user perceptions in implementing centralized EMRs in HIV care.