Abstract: Background: Hospital acquired infections are serious problems in patient care, and adversely affect the mortality and morbidity; despite antimicrobial therapy and advances in supportive care. The environment can serve as a source of infection. The aim of this work is to evaluate contamination of the inanimate hospital environment by bacterial agents, particularly Staphylococcus aureus and Pseudomonas aeruginosa; and to determine the susceptibility of the bacterial isolates to various antibiotics.