Abstract: Altogether 700 segments from 15 plants of Acalypha indica Linn. were collected from Karnatak University Botanical Garden; Dharwad. And they were screened for the presence of endophytic fungi. A total of 12 fungal species viz, Aspergillus candidus Link ex. Fries., Aspergillus flavipes Bainer and Sartory. Aspergillus niger Tiegh., Aureobasidium pullulans (de Bary) Arnaud. Les., Bipolaris nodulosa (Bert and Curt. ex. Sacc.) Shoemaker., Cladosporium epiphyllum Person., Cunninghamella blacksleeana Lender., Fusarium heterosporum., Fusarium oxysporum Schlechtendahl., Penicillum purpurogenum stoll., Rhizopus nigricans Ehrenberg., Unidentified I isolated and identified based on the morphology of the fungal culture and spores with the help of manuals. The present investigation was the diversity of endophytes and colonization frequency was more dominated in host leaves had been determined. Importance of endophytes in medicinal plant has been discussed.