IJSRP, Volume 4, Issue 4, April 2014 Edition [ISSN 2250-3153]
Jayarama Reddy and Jeldi Hemachandran
A comparative evaluation of the antidiabetic and hypoglycaemic potential of the extracts from different plant parts of Musa paradisiaca (MP) was carried out in vitro. MP extracts were prepared sequentially with methanol, chloroform and petroleum ether. A comparison was made between the action of extracts from different parts. Different concentrations of each extract were made by using phosphate buffer and subjected to α-amylase and α-glucosidase inhibitory assays using maltose and 2-Chloro-4-Nitrophenyl-α-Maltotrioside as substrates respectively. Using this method, the percentage of α-amylase inhibitory activities of each extract were calculated. The enzymes were extracted from the sheep intestines. The absorbencies ware read at 595 nm and 405nm respectively using spectrophotometer. Methanol extracts were found to show higher inhibition.