Abstract: Enzymes are biocatalysts obtained from plants, animals and microorganism. Microbial enzymes are becoming important for its technical and economical advantages .Various kinds of microorganisms in nature degrade different type of proteins indicating the Proteolytic capabilities of microorganism. About 80% of enzymes produced annually are simple hydrolytic enzymes, of which 60% are proteases. Different bacteria, fungi and actinomycetes are the major sources of microbial proteases. Extracellular proteases have high commercial value and multiple application in detergent, food, diary, pharmaceutical, leather, diagnostic, waste management and silver recovery industries. Here we consider the extra cellular protease which is secreted by Actinomycetes Sp. This enzyme is free from most of the toxic agents. And it is considered as a safe/suitable protease for the oral medication purposes. Here we find the best suit Actinomycetes strain which yield high protease, and its suitable Ph and the medium (Plate assay method). Then we find the high Protease production in different SSF and LSF. Thereafter we discuss the protease isolation methods, conformation and their application in the medical and industrial fields.