Abstract: Dengue fever (DF) is globally emerging mosquito born viral disease. Pakistan is at high risk of dengue fever due to unavailability of vaccine, crowded cities, inadequate sanitation, unsafe water and large number of refugees. The present study was intended to investigate the epidemiology of DF in Faisalabad, Pakistan. This was a retrospective study to measure the frequency and distribution of the disease. Total 1509 cases of Dengue fever were admitted in Allied Hospital, Faisalabad during 6 months. Case-control epidemiological study was also conducted to identify some of the risk factors of the disease. For this purpose cases (n = 200) and control (n = 200) were confirmed through identification of antibodies against Dengue virus (immunoglobulin G) in the serum.