Abstract: Chronic tension-type headache (CTTH) differs from the episodic form not only in frequency but also with respect to pathophysiology, lack of effect to most treatment strategies, frequent medication overuse, more disability, and higher socioeconomic costs. The purpose of this study is to see the effect of amitriptyline on TNF-α, IL-1, and IL-6 serum levels in CTTH patients and its correlation with pain severity. Method : This research was done at the Adam Malik Hospital and Bukit Barisan Army Hospital Medan, Indonesia from January 2013 - June 2014 and approved by the Ethics Committee for Health Research School of Medicine in University of Sumatera Utara. The subjects were recruited consecutively from study population. Diagnosis of CTTH was based on the diagnostic criteria as stated in the ICH X. We assessed pain severity by using the Numeric Rating Scale ( NRS ) for pain. Venous blood was taken to measure serum levels of TNF-α , IL-1, and IL-6. After subjetcs were given amitriptyline 25 mg once daily in the evening for ten days, NRS scores were reassessed and the second measurements of these cytokines serum level were done.