Abstract: A total of 23,007 clinical samples, 2,001 bacterial isolate were identified as Escherichia coli, of which 205 strains were haemolytic. On biotyping, indole test was found positive in 99.52% strains. Glucose, maltose, trehalose were fermented by all strains. Dulcitol was fermented by 80.98% strains. Raffinose, sucrose, adonitol and inositol were fermented by 61.96%, 49.76%, 35.13% and 34.15% strains respectively. Lysine, ornithine and arginine were decarboxylated / hydrolyzed by 90.74%, 82.44% and 22.44% strains respectively. Serotypability was 82.9% and 51 serogroups were detected. 35.14% belonged to 3 serotypes (O6, O2 & O4). Resistogram typing showed that most of the strains were resistant to Boric acid, Malachite green and Cetrimide. These strains fell into 57 different resistotypes. Antibiogram revealed a higher degree of multiple drug resistance (53.75%).