Abstract: Pomegranate is a curative plant from ancient times which belongs to punicaceae and Lythraceae family. This plant is developed as small trees or shrubs in several countries like Iran, Iraq, Afghanistan, Pakistan, India, Russia and Mediterranean area. From the peel extract the tests were carried out to find the presence of the following chemical compounds such as carbohydrates, reducing sugars, glycosides proteins, amino acids, phenolic compounds, tannins, alkaloids, flavonoids, saponins, sterols,etc. This paper shows a comparative report of the screening test of pomegranate peel using crude, hydro alcoholic extract and In vitro antimicrobial activity.