Abstract: The increase in diabetic population of the world is among the top ten causes of death, with diabetes always high on the list of causes of death. The eating habits and patterns of the diabetic patients are the main causes for pour glycemic control. It is reported in epidemiological studies that high fiber dietary intake can significantly reduce the incidence of diabetes. The study was undertaken to evaluate acceptability and glycemic response of bottle gourd pulp powder (BGPP) enriched biscuits against standard wheat biscuits. Normal healthy subjects (n=7) and prediabetic subjects (n=7) were included in the study. Blood glucose values at 0, 30, 60, 90 & 120 minutes indicated significant difference in incremental area under the curve (IAUC) of glucose and two varieties of biscuits in prediabetic subjects as well as in normal healthy subjects. The mean IAUC of BGPP enriched Biscuits (p=<0.001) was significantly lower than that of glucose and Wheat flour Biscuits (p=<0.001) in normal as well as prediabetic subjects.