Abstract: The noble metal gold and silver nanoparticles have been successfully prepared using a bio-derived product-Active Manuka honey 20+ UMFR. Manuka honey is well recognized for its beneficial properties over and above mixed flora honey for use in wound treatment. Having been found to offer effective antimicrobial, anti-inflammatory and debridement properties as a dressing, it is only the medical grade Active Manuka honey that should be used in open wounds. A high UMFR grading above 10+ is recommended for wounds. The formation of silver and gold nanoparticles has been confirmed using UV–vis, SEM and FTIR analysis and its antibacterial activity was evaluated against Enterococcus faecalis, Staphylococcus aureus and Escherichia coli. Silver and gold nanoparticles showed a clear well defined inhibition zone compared to control. The results suggest the stabilized and reduced molecules of silver and gold nanoparticles may act as an effective antibacterial agent for wound healing.