Abstract: The regenerative potential in the root explants of in vivo grown Cattleya ’Almakee’ plants largely depend on the location of isolated explants from the main root and on the level&type of the growth regulators.Regeneration is affected by polarity all along the root axis of root.The regeneration is of basipetal gradient.The proximal explants responded to the presence of cytokinins(BAP/KN)in Mitra et al.,1976 medium.The effect of cytokinins was accentuated in the additional presence of NAA,whereas the distal explants failed to regenerate.The regenerated plantlets were accilimatized &transfered to pots filled with moss, pine bark, brick &charcoal pieces (2:4:1:1) with 90% survival.