Abstract: Monoclonal antibodies were discovered by Kohler and Milstein in early seventies this discovery leads to a huge revolution in immunology field. In addition to this Kohler and Milstein along with Niels Kaj Jerne were awarded noble prize in 1984. There are various production methods of monoclonal antibodies which includes hybridoma technique which is the most traditional method, phage display, transgenic mouse method also production of monoclonal antibodies from plants this procedure is under development. Hybridoma technique is considered to be the traditional or conventional technique which was used for the first MAb production in 1975.