Abstract: The wound healing component consists of albumin, collagen, omega 3 and omega 6. These components become active substances in ointments used for wound healing. Ointment is a semisolid preparation that is soft, easy to apply, and is used as an external medicine on the skin. The source of albumin is snakehead fish, a source of collagen is grouper skin, omega 3 from flaxseed oil, and omega 6 from black cumin oil. The aim of this study was to obtain the optimal collagen concentration in making the best ointment.