Abstract: Background: The prognosis of breast cancer is dependent on several clinicopathological factors and molecular markers. Among these, angiogenesis is required for growth, invasion and metastasis of tumor. Microvessel density (MVD) assessment aids to quantify angiogenesis which was measured by Haematoxylin and eosin (H&E), Vascular Endothelial Growth Factor (VEGF) and CD31 in the present study, with an objective to find the best stain among the three.