Abstract: Amla is an amazing gift of nature . It has good antimicrobial property . I treated cotton fabric with amla juice by pad batch method in acidic condition . The antimicrobial activity on the cotton fabric was evaluated against AATCC 147 method . . I found there is antimicrobial activity on the fabric after doing test . The aim of the project was to check the antimicrobial activity of cotton fabric after application of amla juice in the fabric.