Abstract: Santalum album commonly known as Sandalwood is used traditionally for health and wellness. It is an evergreen and hemi-parasitic tree and has a long history in Indian religious rituals and traditional Chinese medicine. In the present study we were investigated the antibacterial activity of leaf and fruit extract of Santalum album. The antimicrobial activity of aqueous extract leaf and fruit of Santalum album was performed against gram negative and gram positive bacteria.