Abstract: Several medicinal plants have been used in traditional health care systems to heal wounds. Wound infecting bacteria are one of the major factors that delays or prevent wound healing. In the present study, three medicinal plants those have been widely used in Siddha medicine to treat wounds namely, Erythrina variegata, Tamarindus indica and Datura metel were tested for their antibacterial activity against standard bacterial isolates which isolated from different sources including wounds, viz. Staphylococcus aureus subsp. aureus (ATCC® 29213™), Pseudomonas aeruginosa (ATCC® 25668™), Escherichia coli (ATCC® 25922™) and Enterococcus faecalis (ATCC® 29212™).