Abstract: The present studies has been done on investigation of Karnal bunt in wheatproduced in South WesternPunjab.To know the status of wheat seed,340 wheat samples were collected from 17different grain markets belongs to 9 districts of Punjab.The major five Varieties of wheat i.e. HD 2967, HD 3086, HD2733, PBW 502 and PBW 725were collected from selected area. The results shows Karnal Bunt disease infection was ranged from 0 to 14.2% in south western Punjab. The most susceptible wheat varieties were HD 2967and HD 3086, showing 5.0% and 14.2% of infestation respectively. The minimum incidence of Karnal Bunt was observed on the varieties PBW 725 (0.81% infection) and HD 2733 (2.41% infection). Whereas PBW 502 was Karnal Bunt free.It was also found that Karnal Bunt was least in Abohardistrict whereas Fazilka district wastotally disease - free.