International Journal of Scientific and Research Publications

IJSRP, Volume 2, Issue 8, August 2012 Edition [ISSN 2250-3153]


Analysis of Compost Parameters and a Molecular Approach on its Thermophilic Microflora
      Jelin Ilayaraja. J, Dhanarajan. M.S, Mariappan.V
Abstract: The present study deals on factors and parameters influencing composting. The temperature , pH, ash, moisture, carbon and nitrogen content estimated at every 15 days interval states that composting is high during thermophilic phase than mesophilic phase. The microbial loads of bacteria were estimated by SPC method in PBYG medium. charecterization was done by biochemical analysis, thermotolerance assay and by molecular methods. Amplification of 16s rRNA gene sequence and universal eubacterial primers 27F and 1492 R to give a product of approximately 1500 bp in length was amplified by PCR. Sequencing software windows VQ2, gene codes were used to assemble the fragment. The sequence data was analyzed by comparing in gene bank database using Basic local Alignment Search Tool (BLAST 2.2.1.6) in a nucleotide to nucleotide search of 16s rRNA gene sequence from type strain and species were identified as thermophilic Bacillus pumilis and Pseudomonas stutzeri showing 93% and 98% homology respectively.

Reference this Research Paper (copy & paste below code):

Jelin Ilayaraja. J, Dhanarajan. M.S, Mariappan.V (2018); Analysis of Compost Parameters and a Molecular Approach on its Thermophilic Microflora; Int J Sci Res Publ 2(8) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0812.php?rp=P0733
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