Abstract: Previous different studies in India reported that, transmission of infection to patients occurs through two general routes, airborne and contact. Air is a major source of infection because air within the hospital may act as a reservoir of pathogenic microorganisms. The present study was carried out to determine the degree of “Hospital Infection Control” in different locations of Indraprastha Apollo Hospital New Delhi including OT, IPD, CSSD, MICU, NICU, Kitchen, using different clinical specimens mainly Air sample, Environmental swabs and Hands swabs, from Jan 09 to Jun 09.