Abstract: Knowledge level on HIV/AIDS is still of paramount importance to every individual across the world. This is because HIV virus is still a killer disease and no vaccine has been discovered to date A baseline survey was conducted among 265 participants out of a total population of 379 in one of the Universities in Kenya (Pwani University) on staff knowledge level on HIV and AIDS. Questionnaires and interview schedules were used for data collection. Both qualitative and quantitative data analysis technique was used to analyze data. The study found out that staff HIV knowledge level varied on various areas tested, Knowledge level on HIV infection and transmission was 94.3%, CI 95.86-100, p < 0.05 and protection was 95.7%. Due to varied response to and low knowledge level in some areas, further studies on prevention is still necessary.