Abstract: The objective of this research was to know the influence of Entrepreneurial Competencies and External Environmental Dynamism, to Organizational Ambidexterity and its implication on Sustainable Competitive Advantage and impact on Business Performance. The research method was quantitative through survey research approached by causal comparative. The sample technique was probability sample with sampling multistage methods in number of respondents was 180 of logistics service companies in Jakarta, Indonesia. The analysis technique used Structural Equation Modeling (SEM) by software Lisrel 9.1. The result of this research showed the relations and strong impact between the whole variables.