Abstract: The efficient cellulase producing bacteria was isolated from the soil of sacred groove and identified as P. aeruginosa SG21 by 16S rRNA sequencing. The objective of this study is to perform biochemical testing, antibiotic sensitivity testing and optimizing the growth conditions of the bacteria P. aeruginosa SG21. The culture conditions like pH, temperature, carbon sources, and nitrogen sources were optimized. The optimum conditions found for cellulase production are 35 and 40°C at pH 6-10 with CMC as carbon source and urea as nitrogen source.