International Journal of Scientific and Research Publications

IJSRP, Volume 6, Issue 5, May 2016 Edition [ISSN 2250-3153]


Amino Acid Determination of the Dominant Protein Isolated from Fertilized, Corticated Ascaris lumbricoides Egg
      De Guzman, Ana Isabella, Sevilla, Alyssa Denise, Bernabe, Adam Lesner, Baustista, Carmela, Yanga, Aedrick, De Ocampo, Yves Aaron, Martin I, Gregorio, Guevarra Jr., Leonardo
Abstract: Ascariasis is a neglected tropical disease (NTD) affecting one billion people worldwide. The most common laboratory diagnosis is still through the microscopic identification of Ascaris lumbricoides eggs from stool sample; thus, there is a need to establish a gold standard for its identification. In line with this, investigation of the amino acid content of the protein coat was done for possible development of rapid diagnostic tests. Micro-Bradford assay of the supernatants after decortication using Phosphate-Buffered Saline with Tween 20 revealed increasing absorbance suggestive of increasing protein content (r=0.71). Results of the High-Performance Liquid Chromatography exhibited peaks belonging to arginine and tyrosine. Moreover, these amino acids recovered are present in proteins which are inhibitors of mammalian trypsin and chymotrypsin.

Reference this Research Paper (copy & paste below code):

De Guzman, Ana Isabella, Sevilla, Alyssa Denise, Bernabe, Adam Lesner, Baustista, Carmela, Yanga, Aedrick, De Ocampo, Yves Aaron, Martin I, Gregorio, Guevarra Jr., Leonardo (2018); Amino Acid Determination of the Dominant Protein Isolated from Fertilized, Corticated Ascaris lumbricoides Egg; Int J Sci Res Publ 6(5) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0516.php?rp=P535413
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