Abstract: The focus of this research was to find the effect of Rhamnus (Rhamnus cathartica) and Henna (Lawsonia inermis) an important medicinal plant extracts, phytochemical constituents and role on hair growth and wound healing. The extracts of the selected medicinal plants are prepared through two different methods namely soxhalation and maceration to determine the effective method of extraction.Phytochemical screening of both the extracts were carried out to check the preliminary phytoconstituents followed by HPLC analysis is performed to determine the quantity of phenol and flavanoids. Antimicrobial study was studied on selective microorganisms and in vivo study of extracts was carried out on mice with parameters like hair growth and wound healing. The Results of phytochemical screening clearly shows that presence of phenols(++) and flavanoids(+++) in all the tested seed and leaf extracts of Rhamnus while flavanoids are absent in henna extracts. The amount of phenol that recognized in HPLC varies according to the plant materials used and type of extraction methods, this is an indicator of extraction method also plays a role in presence or absence of particular constituent. In addition the invivo study on mice wound healing and hair growth study also showed a clear result of faster recovery from wound and hair growth.