Abstract: Artocarpus heterophyllus Lam (Jackfruit) which belongs to the Moraceae family , is believed to be indigenous to the south western rain forest of India. There exists a lot of variability among jack trees since most of them are raised from seedlings. A survey has been conducted in Kuttanad region to find out promising jack types during 2010-12 and selected six superior trees based on physico-chemical characters and organoleptic properties. Hence a study was undertaken to realize the genetic relationship among these jackfruit selections during 2012-2014, using RAPD technique. DNA was isolated from young leaves of six A. heterophyllus selections . Out of the thirty RAPD primers used for the analysis only ten produced maximum reproducible polymorphic bands (OPA-1, OPA-2, OPA-4, OPA9, OPC7, OPD19, OPN-05, OPM- 16, OPG-03 and OPG-10) . The primer OPA-1 gave the maximum number of bands and OPN-05 produced least. An UPGMA dendrogram was constructed and the statistical analysis was done using NTSYSPC-2.02i.