Abstract: We investigated the effects of serum starvation and cell density expression of Vascular endothelial growth factor (VEGF), Basic fibroblast growth factor (bFGF) and Angiogenin by used PC3M cells, we demonstrated that low density increase the expression of VEGF and bFGF but no effects on Angiogenin in the mRNA levels, while no deferent in high density. However the VEGF and bFGF was decrease when the cells was starved 24h but no effects on angiogenin in the mRNA levels, also We transfected the VEGF gene in the sense orientation into PC3M cells, and obtained stable VEGF overexpressing transfectants. The bFGF and angiogenin was detected ,and we obtain the VEGF up regulate increase of bFGF expression level, and negative correlation was found between up regulate VEGF and angiogenin expression level.