International Journal of Scientific and Research Publications

IJSRP, Volume 5, Issue 4, April 2015 Edition [ISSN 2250-3153]


Effect of serum starvation and cell density in the expression of VEGF, bFGF and Angiogenin, and also the effect of VEGF up regulate in the expression of bFGF and Angiogenin
      Mohnad Abdalla , Wafa Ali Eltayb
Abstract: We investigated the effects of serum starvation and cell density expression of Vascular endothelial growth factor (VEGF), Basic fibroblast growth factor (bFGF) and Angiogenin by used PC3M cells, we demonstrated that low density increase the expression of VEGF and bFGF but no effects on Angiogenin in the mRNA levels, while no deferent in high density. However the VEGF and bFGF was decrease when the cells was starved 24h but no effects on angiogenin in the mRNA levels, also We transfected the VEGF gene in the sense orientation into PC3M cells, and obtained stable VEGF overexpressing transfectants. The bFGF and angiogenin was detected ,and we obtain the VEGF up regulate increase of bFGF expression level, and negative correlation was found between up regulate VEGF and angiogenin expression level.

Reference this Research Paper (copy & paste below code):

Mohnad Abdalla , Wafa Ali Eltayb (2018); Effect of serum starvation and cell density in the expression of VEGF, bFGF and Angiogenin, and also the effect of VEGF up regulate in the expression of bFGF and Angiogenin; Int J Sci Res Publ 5(4) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0415.php?rp=P403928
©️ Copyright 2011-2022 IJSRP - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.