International Journal of Scientific and Research Publications

IJSRP, Volume 3, Issue 4, April 2013 Edition [ISSN 2250-3153]


Analysis & Trouble Shooting of the Contaminations, Arised in North Indian Zones during Plant Tissue Culture
      Pushpendra Singh, Madhulika Singh, Sujeet Kumar Singh
Abstract: In the present investigation Jatropha and pea plant leafs was used, to germinated the plant by artificial condition through plant tissue culture method. We have taken three methods Method A, B & C to germinate callus, seed, bud and stem. 30 Sample were taken to analyze. In Method A 60% samples were contaminated (fungus and bacteria) and 20% was shown growth. In Method B 66% was contaminated by fungus and 19% were contaminated by bacteria and only 15% are shown growth. In Method C 85% samples were contaminated by fungus and bacteria, only 15 % samples was show growth. The study shows most of all contamination was due to Fungus, a very less contamination were due to bacteria. Among three method used with different Sterilization process. Method A was best suited for Callus Culture, Method B for Seed Culture & Method C for Stem & Bud Culture.

Reference this Research Paper (copy & paste below code):

Pushpendra Singh, Madhulika Singh, Sujeet Kumar Singh (2018); Analysis & Trouble Shooting of the Contaminations, Arised in North Indian Zones during Plant Tissue Culture; Int J Sci Res Publ 3(4) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0413.php?rp=P16990
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