Abstract: In the present investigation Jatropha and pea plant leafs was used, to germinated the plant by artificial condition through plant tissue culture method. We have taken three methods Method A, B & C to germinate callus, seed, bud and stem. 30 Sample were taken to analyze. In Method A 60% samples were contaminated (fungus and bacteria) and 20% was shown growth. In Method B 66% was contaminated by fungus and 19% were contaminated by bacteria and only 15% are shown growth. In Method C 85% samples were contaminated by fungus and bacteria, only 15 % samples was show growth. The study shows most of all contamination was due to Fungus, a very less contamination were due to bacteria. Among three method used with different Sterilization process. Method A was best suited for Callus Culture, Method B for Seed Culture & Method C for Stem & Bud Culture.