In-Vitro Transcription and Transfection of HCV Genomic Replicons

IJSRP, Volume 9, Issue 3, March 2019 Edition [ISSN 2250-3153]

In-Vitro Transcription and Transfection of HCV Genomic Replicons

Binod Gupta

Abstract: Introduction: Hepatitis C virus (HCV) is a positive stranded RNA virus that causes acute and chronic hepatitis and hepatocellular carcinoma. Aims & Objectives: The study was conducted to establish the transfection of Huh 7.5 derived cell lines with In-vitro transcript of HCV pF6/JFH-1 for production of infectious virus particles in naïve Huh 7.5 cells, its detection by RT-PCR

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Binod Gupta (2019); In-Vitro Transcription and Transfection of HCV Genomic Replicons; International Journal of Scientific and Research Publications (IJSRP) 9(3) (ISSN: 2250-3153), DOI: http://dx.doi.org/10.29322/IJSRP.9.03.2019.p8764