International Journal of Scientific and Research Publications

IJSRP, Volume 5, Issue 3, March 2015 Edition [ISSN 2250-3153]


Optimization of a manual and rapid Silica-based DNA Extraction Method: Applied to Human papillomavirus detection using fresh Cervical Biopsies Samples
      Luc Magloire Anicet BOUMBA, Samira ZOA ASSOUMOU , Donatien MOUKASSA, Nadia Touil, Omar MESTOUI, Abdelkrim KHATTABI, Lahoucine HILALI, Moulay Mustapha ENNAJI
Abstract: Since the advent of the polymerase chain reaction in 1985, the extraction of DNA has become an important step in the diagnosis of certain diseases. To do this, manual extraction methods remain indispensable alternative for limited resources laboratories in developing countries because of the cost of commercially available kits. The main objective in this study was to test and optimize a fast, non-toxical, high reliable and low cost silica-based method of viral HPV-DNA extraction protocol from tissue specimens.

Reference this Research Paper (copy & paste below code):

Luc Magloire Anicet BOUMBA, Samira ZOA ASSOUMOU , Donatien MOUKASSA, Nadia Touil, Omar MESTOUI, Abdelkrim KHATTABI, Lahoucine HILALI, Moulay Mustapha ENNAJI (2018); Optimization of a manual and rapid Silica-based DNA Extraction Method: Applied to Human papillomavirus detection using fresh Cervical Biopsies Samples; Int J Sci Res Publ 5(3) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0315.php?rp=P393876
©️ Copyright 2011-2022 IJSRP - All rights reserved. Use of this web site signifies your agreement to the terms and conditions.