Abstract: Tribal’s and their symbiotic relationship with forest and their surrounding environment has been seen from the ancient time. Their relationship with forest has crossed many centuries and forest maintained their existence until now. The nature of any type of new project in the forest area has negative and positive impacts and widen of these impacts can be minimal to significant in terms of biological environment and dependency of tribal habitat on the forest area. Therefore, mining operations essentially involves many significant impacts on deforestation, habitat destruction and biodiversity. Present study was based on study of biological environment and tribal habitat dependency on it in various sectors in the context of Ambaji Multimetal Mine project. Study of flora and fauna and tribal habitat was carried out to observe the interrelationship of these two.