IJSRP, Volume 6, Issue 2, February 2016 Edition [ISSN 2250-3153]
Dr. Shweta Mathur, Dr. Asha Goswami
In present study cotyledonary nodal segments and nodal shoot segments were used for regeneration. Nodal segment explant as well as cotyledonary nodal explant were incubated on basal MS medium and showed very poor percentage of shooting, explants inoculated on MS medium supplemented with either BAP (0.1-3.0 mg/L) or Kn (0.1-3.0 mg/L) alone produced multiple shoots. Best response of multiple shoot induction was obtained on MS medium supplemented with both BAP (2.0 mg/L) and NAA (0.5 mg/L) and the least responsive combination for multiple shooting in nodal segment as well as cotyledonary nodal explant was 0.1 mg/L Kn. MS medium supplemented with cytokinins alone (BAP or Kn) produced shoots in both the explants but MS medium supplemented with cytokinins (BAP or Kn) + auxins (NAA or IBA) both, produced more percent of shoot regeneration as well as number of shoots / culture. Increased rate of multiplication or shoot induction in cultures supplemented with cytokinin (BAP or KN) and auxin (NAA or IBA) may be due to enhanced rate of cell division by cytokinin (BAP and Kn).