Abstract: The study focuses on examining the effect of exercise on the cardiovascular system. A sample size of twenty athletes and non-athletes were chosen of which we have five regular or seasoned athletes who were male, five regular or seasoned athletes who were female, five non regular athletes male and five non regular female athletes. These non-regular or untrained athletes and trained athletes were put together to have ten weeks session of training so as to see the effect that exercise will have on their cardiac output. After this exercise, the data generated were analyzed using simple descriptive statistics technique and physiological calculation of the stroke volume and the Heart rate to arrive at the cardiac output. It was realized that the cardiac output of both groups varied as some had a reduced hate rate while others had an increase in heart rate that leads to the various results on the cardiac output.