Abstract: Separator gels are composed of polymer gels contained in a tube which are used to separate serum from formed elements for various diagnostic tests. An alternative serum separator gel, 0.25 g/mL of Ipomoea batatas, was used in total cholesterol and creatinine test determination. Results show that Ipomoea batatas starch can be used as an alternative serum separator gel for the determination of creatinine and total cholesterol as all the samples in the alternative serum successfully separated the serum from the formed elements. Despite its ability to lower the cholesterol levels, Ipomoea batatas can be used as a serum separator gel for the total cholesterol determination as there is a stronger agreement [ρc = 0.84] in the total cholesterol levels of serum separator tubes and Ipomoea batatas starch serum separator tubes, as compared to the agreement [ρc = 0.53] in the creatinine levels.