Abstract: Angiogenesis that plays important roles in a variety of physiological processes is strictly delimited and finely tuned by a balance of pro-angiogenic and anti-angiogenic factors. Angiogenesis is essential for viability, growth, invasion and metastasis of tumors. The chorioallantoic membrane model is considered to be a valuable method for investigation of anti-angiogenic effects of drugs and other substances. The aim of the present study is to investigate the anti-angiogenic effects of a calcium channel blocker (diltiazem), a tyrosine kinase inhibitor (imatinib), and a vascular endothelial growth factor inhibitor (bevacizumab) with the means of the chicken chorioallantoic membrane (CAM) model.