Abstract: Endophytes are the microbes which are invading in the host plant tissues and are believed to produce the similar metabolites as that of the plant tissue. The present study was performed for isolation, identification and determination of endophytes from leaves, stem and roots of Calotropis procera (Aak/Madar) antimicrobial potential against the dreadful pathogens and drug resistant strains. It is already reported that, Calotropis procera plays an important role in improving soil fertility and improved soil water holding capacity.