Rapid, Selective, Rugged and High throughput Simultaneous Method Development and Validation of Pioglitazone and its Metabolites, M III and M IV in Plasma Using 96 well Plate Solid Phase Extraction and Liquid Chromatography Coupled with Tandem Mass Spectrometry

IJSRP, Volume 6, Issue 2, February 2016 Edition [ISSN 2250-3153]

Tangudu Nagabhusana Rao; Guntuku Girija Sankar; Lade Jyothi Rani

Abstract: A high throughput, sensitive, selective, and rugged liquid chromatography coupled with mass spectrometry (LC-MS/MS) method for the quantification of Pioglitazone and its active metabolites, M III and M IV, in human plasma was developed and validated. The analytes were extracted from plasma by solid phase extraction technique using Waters Oasis HLB 96 well plate 30 µm (10 mg). Isocratic elution of Pioglitazone and its metabolites were achieved in 5.5 min

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Tangudu Nagabhusana Rao; Guntuku Girija Sankar; Lade Jyothi Rani (2018); Rapid, Selective, Rugged and High throughput Simultaneous Method Development and Validation of Pioglitazone and its Metabolites, M III and M IV in Plasma Using 96 well Plate Solid Phase Extraction and Liquid Chromatography Coupled with Tandem Mass Spectrometry; Int J Sci Res Publ 6(2) (ISSN: 2250-3153). http://www.ijsrp.org/research-paper-0216.php?rp=P505097