Abstract: The aim of this work was to investigate and compare the phytochemical screening and antimicrobial activities of different crude extracts from leaves, barks and roots of Khaya senegalensis. Two different organic solvents including methanol and ethanol were used to prepare the crude extracts from the fresh and dry leaves, barks and roots. Antimicrobial activities of different crude extracts from dry and fresh leaves of Khaya senegalensis were determined by agar disc diffusion method with minor modification. In vitro phytochemical screening for all crude extracts from all leaves, barks and the roots were tested and shown positive result for all the compounds. The methanol crude extract and its derived fractions from leaves, barks and roots showed small and moderate antibacterial potential with one gram positive (Staphylococcus aureus) and three gram negative (Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa) bacteria in the range of 0–21%. In conclusion, all organic crude extracts from leaves, barks and roots could be used as potential sources of new antimicrobial properties.