Abstract: The seeds of horsegram (Macrotyloma uniflorum (Lam.) Verdc) cv. Dapoli Kulthi- 1 were subjected to gamma radiation (100, 200, 300 and 400Gy), EMS (0.2, 0.3, 0.4 and 0.5 %) and combination treatments. The mutations affecting gross morphological changes in growth and yield characters such as plant habit, flowering, pod morphology, maturity and seed yield were scored as quantitative characters. The micromutations at the population level can be easily detected in the form of increased variations for quantitative traits in the segregation of mutagen treated populations. Micromutations can alter morphophysiological characters hence they are of a particular interest to the plant breeders. Both the mutagens, gamma radiations and EMS proved to be very effective to induce variability in quantitative traits like plant height, primary branches per plant, days required for first flowering and first pod maturity, number of pods per plant, pod length, number of seeds per pod, 1000 seed weight and yield per plant in M2 and M3 generations. In present investigation positive as well as negative impact on quantitative traits was recorded.