Abstract: Panchagavya ghrita [PGG] is one of the medicated ghee formulations, contains five cow products; cow ghee, cow milk, cow urine, cow dung juice and cow curd. PGG was prepared as per established SOP and compared with standard values. Nootropic activity of Panchagavya Ghrita (PGG) was studied using Diazepam induced amnesia in mice and Morris Water Maze test in rat model. PGG also reversed successfully the amnesia induced by Diazepam (1mg/kg, i.p.). PGG was administered in three dose levels of PGG as X/2 (2.5gm/kg), X (5gm/kg) and 2X (10gm/kg) in mice and X/2 (1.75gm/kg), X (3.5gm/kg) and 2X (7.0gm/kg) in rats.