Abstract: This study aimed to investigate the level of mathematics anxiety and the relationship between mathematics anxiety and mathematics performance among undergraduate students of Payame Noor university (PNU) in Hamedan, Iran. In particular, the study investigated the relationship between mathematics anxiety and gender, and field of study. The sample of the study consisted of 275 (162 women and 113 men) undergraduate students of various fields of study. To measure the mathematics anxiety level, the participants responded to the 24 Items of The Revised Mathematics Anxiety Rating Scale (RMARS), which consisted of learning mathematics anxiety and mathematics evaluation anxiety subscales. The data were analyzed by One-way ANOVA and independent-samples t- tests using the Statistical Procedures for Social Sciences (SPSS 11.5). The results showed significant differences between men and women’s evaluation anxiety and no significant difference was observed concerning field of study. Also, there is a significant correlation between high level anxiety and low academic performance.