Abstract: Ventilago maderaspatana is a medicinal herb belonging to family Rhamnaceae. This herb is used in traditional medicine for Kapha, Dyspepsia, Colic disorder, Leprosy, Skin diseases and general disability. In our previous work we have noticed that methanolic extract of stem bark of Ventilago maderaspatana have exhibited strong antioxidant and significant antibiotic activity on gram negative bacteria and Candida albicans and the major phytochemicals phytosterols, flavonoids and alkaloids were noticed in bioactive extractive. During our present investigation on isolation of active constituents from bioactive fraction using chromatographic methods, we have isolated a pure compound designated as (E)-6-(3,4-dihydroxy-2-methyl-4-(2,6,6-trimethylcyclohex-2-enyl)but-1-enyl)-7-methoxy-2H-chromen-2-one, a novel coumarin derivative from analysis of UV, IR, proton NMR and Mass spectral data .We report this compound as a new natural product .