Abstract: Curcuma amada Roxb, of Zingiberaceae is an important active spice used in traditional system of medicine. They also used in the manufacture of pickles, chutney, salad and jam. Phosphomolybdenam method was used to measure the total antioxidant capacity (TAC) of C. amada ethanolic rhizome extract (CAEREt). The free radical scavenging capacity of CAEREt was also carried out by ABTS and DPPH methods. The cytotoxicity effect of CAEREt was determined by Trypan blue dye exclusion method and MTT assay using two different cell lines such as Dalton Lyphoma Ascites (DLA) and Human Cervical Cancer Cell Line (HeLa). The results showed that the rhizome extract of C. amada rich in phytocompounds and have exhibited better antioxidant and cytotoxicity properties.